#BIORAD WESTERN BLOT PROTEIN LADDER PLUS#

Place the gel in 1x transfer buffer for 1015 min. Transferring the protein from the gel to the membrane 1. For details, please refer to the Protein Blotting Guide, bulletin 2895. No conjugate is needed for fluorescent blot detection. A 420 gradient gel separates proteins of all sizes very well. The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add. Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3, 0.01% bromophenol blue.įluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on. I want to load 50 ug/20 ul/well of SDS-PAGE for Western blot and I have protein concentrations 6.18, 4.9, 5.76, 6.53, 4.43, 5.83, 5.11& 7.46 ug/ul. Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa.Molecular weight sizing of proteins after blot development.Monitoring blot transfer efficiency with easy orientation via multicolor standards.These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.

#BIORAD WESTERN BLOT PROTEIN LADDER PLUS#

Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. Ladder (Thermo Fisher Scientific) PBS (phosphate-buffered saline) Low-fat milk powder Bovine Serum Albumin (BSA) Tween 20 (Sigma. LC-MS innovation: Improve analytical accuracy List Price: Your Price: Log in to see your price. 2.5 ml (5 x 500 l), mixture of 12 recombinant proteins (2250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications. Which microbioreactor modules are best for you Precision Plus Protein Dual Xtra Prestained Protein Standards, value pack. Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. Stronger blot persistence provides enhanced: Confidence after western blot processing. Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. Increased brightness makes it easier to: Identify target proteins on the gel or blot. These standards can be detected by fluorescence when excited at appropriate wavelengths.Which solid support is right for your oligo synthesis? Precision Plus Protein WesternC Standards are combination protein standards containing ten prestained, Strep-tagged recombinant proteins, enabling both fluorescent visualization on gels and colorimetric or chemiluminescent western blot detection with StrepTactin-AP or StrepTactin-HRP Conjugate. Improved Dual Color Standards are brighter than the competition on both gels and blots. Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10–250 kD). Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.

Biorad western blot protein ladder